5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Knowledge-enriched characterization of mobile populations. Morphological information and facts from illustrations or photos can increase for the richness of flow cytometry data. By way of example, the determine exhibits an in any other case typical apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Each and every inhabitants to expose morphologically unique features. These insights could not are received from multiplex staining alone.
Whether you’re a novice or a professional, investigate our assets to advance your investigation and know-how in flow cytometry.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The amount of singlets obtainable for analysis improves once the user trains and employs a more accurate design.
Discover new insights into your experimental information by visualizing populations using merged fluorescence and morphology data. Our application interface wizards help downsampling, concatenation (merged files) and dimensionality instruments that are crafted into your software program for an intuitive, streamlined data Evaluation working experience.
"An image just isn't quite really worth 1000 dots, nonetheless it actually helps you to see photos. It is excellent to see photos that correlate with staining. For instance, AnnexinV-constructive cells that are morphologically distinct than healthful cells, or CD14+ monocytes which can be more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it really is illuminating to check out the number of doublets slip by traditional singlet gates, and terrific to validate the position of unanticipated double-good occasions in my analysis."
Optimizing gating approach. Even sturdy guide singlet gating is mistake-vulnerable and continues to be a subjective choice place in Nearly all flow cytometry assays. Imaging can be utilized to substantiate and regulate gates to include only solitary cells of desire.
Our scientists will layout panels to suit your needs as again and again you'll need—free of charge, no product or service purchase essential.
The Attune NxT cytometer is the trustworthy workhorse instrument your lab can rely upon for consistent and trusted overall performance. The Attune CytPix product includes a large-pace brightfield digital camera and AI-driven impression Investigation computer software, with current updates such as equipment learning instruments and an intuitive user interface, enabling Increased information precision, precision, and speedier insights into check here sample biology.
Most likely most significantly, these situations consist of cells of Evidently distinctive phenotypes which could lead on to incorrect conclusions regarding double optimistic functions (particularly in rare populations).
Sound waves help specific mobile alignment, leading to exceptional knowledge quality and ~10X more rapidly sample flow fees.
We Earlier shown the strength of imaging Car or truck-T/Ramos cell interactions. Allow’s have a look at just the inhabitants of greatest desire, the double constructive activities, to learn more. We can easily now use extended morphological parameters (listed here, circularity and skewness of depth) to more analyze the characteristics of those populations and refine gating on these situations, growing facts robustness.
Leverage the power of Sophisticated technological innovation and innovation to speed up your discoveries. Rely on inside the Attune Flow Cytometer loved ones to deliver the dependability, pace, and precision that established your do the job apart.
No clean/no lyse leukocyte Examination with imaging. Cells have been obtained from 24-hour-previous blood by dilution in one mM EDTA ( Specialist UX/UI enables buyers to train AI models for expanded apps. (A) The “Train Design” attribute delivers annotation equipment and true-time design training precision.
At the heart of each and every Attune Flow Cytometer lies our innovative acoustic concentrating know-how, which utilizes audio waves to meticulously align cells inside the flow cell. This modern approach surpasses regular hydrodynamic solutions, causing a lot quicker processing speeds, Remarkable information excellent, and elevated sample throughput.
As the mobile line had Beforehand been analyzed about the Attune CytPix Flow Cytometer, the researchers went back again to the photographs and have been in the position to doc the microbial infection at the very least five days previously.
The Attune CytPix Flow Cytometer allows buyers to visualise samples in the course of acquisition, validate the accuracy of gating approaches, and assistance benefits with trusted documentation for publication needs.
Below, we exhibit that by gating approaches using image-primarily based quantitative parameters can distinguish interacting cells from coincident situations to far more accurately examine interacting cells.